A: NF-κB, JNK and JAK-STAT inhibition prevent LPS- induced iNOS in BV2 cultured alone. BV2 cells were stimulated with LPS in the presence of inhibitors against MEK1 (PD9805, 20 μM), JNK(SP600125, 10 μM), NF-κB (PDTC, 50 μM), JAK-STAT (AG490, 10 μM), p38 MAPK (SB20580, 10 μM) or PI3K (wortmannin, 100 nM). Inhibition of JNK, NF-κB and JAK-STAT reduced NO accumulation, whereas inhibition of MEK1, p38 MAPK and PI3K did not (A). Inhibition of JNK, JAK-STAT and p38 MAPK all protected against LPS-induced toxicity, but inhibition of MEK1, NF-κB or PI3K did not (B). (n = 12 independent observations), *P < 0.05 versus control, #P < 0.05 versus LPS.
Kacimi et al. Journal of Inflammation 2011 8:7 doi:10.1186/1476-9255-8-7