LPS does not affect iNOS expression and cell survival in endothelial cells. bEND.3 cells exposed to 1 μg/ml LPS for 24 h fail to experience any cell death (A, C, D-control, B, E, F-LPS treatment). Shown are trypan blue (A, B), calcein (C, E) and ethidium homodimer (D, F) stains. LPS has no effect on bEND.3 cell viability as assessed by MTT staining (H) and NO generation (G). LPS also fails to induce iNOS protein in bEND.3 cells (I). iNOS protein is readily induced by LPS in BV2 cells as a positive control. Data are representative of 3-5 experiments.
Kacimi et al. Journal of Inflammation 2011 8:7 doi:10.1186/1476-9255-8-7