ELISA analysis of Leishmania-mediated cytokine suppression. Cells were incubated with LPS (100 ng/ml) followed by L. major, L. amazonensis or latex beads at a parasite/bead to host cell ratio of 20:1 (indicated by blue, green and open bars, respectively). At 19 hrs, culture supernatants were taken, centrifuged then assayed for IL-17 production by ELISA as described in Methods. Media only, L. major alone, L. amazonensis alone, latex beads alone and LPS alone (black, gray, purple, brown and red bars, respectively) were used as controls. Data represented are averages of two independent replicate experiments, with error bars as denoted.
Lapara and Kelly Journal of Inflammation 2010 7:8 doi:10.1186/1476-9255-7-8