Figure 3.

Effect of POMx on the MAPKs phosphorylation in PMACI-stimulated KU812 cells. After pretreatment with POMx (20–100 μg/ml) for 1 h at 37°C, KU812 cells (3 × 10⁶ cells ml^-1) were stimulated by PMA (40 nM) plus A23187 (1 μM) for 2 h, then the phosphorylation of JNK (p54/p46), and ERK (p44/42) was determined by Western blot analysis. Image were digitally captured and the band intensities (pixels/band) were obtained using the Un-Scan-It software and are expressed in arbitrary O.D. units. Data shown is cumulative of two experiments and the O.D. values are mean ± SD. *P < 0.001 Vs PMACI alone (P-JNK/ERK-p54), ^#P < 0.001 Vs PMACI alone (P-JNK/ERK-p46).