Figure 1.

Effect of POMx and specific inhibitors for MAPKs and NF-κB on the gene expression of pro-inflammatory cytokines in PMACI-stimulated KU812 cells. KU812 cells (3 × 10⁶ cells ml^-1) were pretreated with POMx (20–100 μg/ml) for 1 h and stimulated by PMA (40 nM) plus A23187 (1 μM) for 4 h. The mRNA expression level of IL-6 (A) and IL-8 (B) was determined by quantitative reverse transcriptase PCR. The concentration of specific inhibitors of ERK (PD98059), JNK (SP600125) and NF-κB (MG-132) used in these studies was 50 μM, 10 μM and 100 μM, respectively. Values shown are Mean ± SD of four independent experiments and differ without a common letter P < 0.01.