Figure 3.

LMP-420 decreases serum and colonic TNF induced by LPS stimulation in vivo. A. Mice were pre-treated with 2 mg LMP-420 i.p. for 16 days. Four hours after the last injection, mice were challenged i.p. with a lethal dose of 0.5 mg LPS then euthanized 2 hrs. later for measurement of TNF levels in serum and in colon tissue lysates prepared at 100 mg tissue/ml. Serum TNF was below the limit of detection (< 10 pg/ml) in control mice not exposed to LPS challenge. *p < 0.05 and **p < 0.001 relative to control LPS-stimulated mice. B. In 2 separate experiments, groups of 5 mice were pretreated with 75 mg/kg LMP-420 given orally in food for 5 days prior to LPS challenge and TNF measurement. Data is presented as % of control value rather than as absolute numbers because mean serum TNF levels after LPS stimulation of control mice differed markedly (706 vs. 1870 pg/ml) in the 2 experiments. * indicates p < 0.05 compared with control.