LMP-420 inhibits production of TNF protein by murine lymphocytes and macrophages. A. A 2 hr pre-treatment with LMP-420 markedly decreases TNF production by thioglycollate-elicited macrophages (MΦ) (n = 3) 24 hrs after exposure to LPS. The IC50 threshold (the concentration that inhibits 50% of the TNF produced) is indicated by the dotted line and is slightly less than 1 μM for this assay. * indicates p ≤ 0.03. B. Pre-treatment with LMP-420 also markedly decreases TNF production by splenocytes (n = 3) 24 hrs after LPS exposure. The IC50 threshold for this assay (dotted line) was slightly less than 1 μM. C. Pre-treatment with LMP-420 also significantly decreased TNF production by splenocytes (n = 8) at both 24 and 48 hrs after stimulation with CD3 antibody. The IC50 for CD3-stimulated T cells at 48 hrs (dotted line) was slightly greater than 1 μM. * indicates p < 0.03 and ** indicates p ≤ 0.003 vs. CD3-stimulated control not exposed to LMP-420.
Hale and Cianciolo Journal of Inflammation 2008 5:4 doi:10.1186/1476-9255-5-4