Research

Possible contribution of endogenous carbon monoxide to the development of allergic rhinitis in guinea pigs

Yu Shaoqing¹ , Zhang Ruxin² , Chen Yinjian³ , Chen Jianqiu¹ , Zhu Chunsheng¹ , Tang Jiangfeng¹ and Li Genhong¹

¹  Department of Otolaryngology, Jinan General Hospital of PLA, Shandong, 250031, PR China

²  Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai, 200040, PR China

³  Department of laboratory medicine, Jinan General Hospital of PLA, Shandong, 250031, PR China

author email corresponding author email

Journal of Inflammation 2008, 5:23doi:10.1186/1476-9255-5-23

Published:	5 December 2008

Abstract

Background

The mechanisms responsible for the development of allergic rhinitis(AR) are not fully understood. The present study was designed to explore the possible roles of carbon monoxide(CO) on the pathogenesis of AR.

Methods

AR guinea pig model was established by nasal ovalbumin sensitization. Twenty-four AR guinea pigs were divided into four groups, 6 in each: Saline control group, AR sensitized group, Hemin treated group, and Zinc protoporphyrin (ZnPP) treated group. The frequency of sneezing and nose rubbing was recorded. Leukocyte infiltration in nasal lavage fluid, serum IgE level and plasma CO were measured. Expression of heme oxygenase-1 (HO-1) mRNA in nasal mucosa was determined by real time RT-PCR, and expression of HO-1 protein was detected by immunohistochemistry.

Results

The frequency of sneezing and nose rubbing, leukocyte infiltration, serum IgE, plasma CO, and HO-1 mRNA levels in sensitized guinea pigs were higher than those of control (P < 0.05). Except for serum IgE level, all above parameters were even higher (P < 0.05) when treated with Hemin, a heme oxygenase-1 inducer; but significantly decreased (P < 0.05) when treated with ZnPP, a heme oxygenase inhibitor. Immunohistochemical results showed that positive staining of HO-1 was present in the lamina of mucosa of sensitized guinea pigs, and there was an increase of HO-1 immunoreactivity with Hemin administration (P < 0.05) and a decrease with ZnPP treatment.

Conclusion

The endogenous CO may take part in the inflammation process of AR and is positively correlated with expression of HO-1 in nasal mucosa. Endogenous CO plays a significant role in the pathogenesis of AR.