Cellular and molecular mechanisms of cigarette smoke-induced lung damage and prevention by vitamin C

Additional file 1:

Identification and characterization of p-benzosemiquinone (p-BSQ). p-BSQ was identified by HPLC and characterized by various physico-chemical analyses, including UV, mass, NMR and ESR spectroscopy. The file contains legends of additional figure 1 (see additional file 2) and legends of additional figure 2 (see additional file 3).

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Additional file 2:

Additional figure 1 depicting HPLC, mass, UV, NMR and ESR spectra.

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Additional file 3:

Additional figure 2 depicting mass spectra of p-BQ-BSA covalent Michael adduct. We performed MALDI-TOF-MS analyses of BSA before and after incubation with p-BSQ or p-BQ at pH 7.4. We observed that incubation of 100 μg of BSA (MW 66,340 Da) with 185 nmoles of p-BQ produced an adduct of MW 68,347 Da, indicating association of 19 nmoles of p-BQ in the BSA molecule. When p-BQ was replaced by p-BSQ, the MW of the product was found to be 67,292 DA, indicating the addition of 9 nmoles of p-BQ.

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