Figure 3.

A, Immunoblots of the DNP-derivatives of lung proteins of guinea pigs exposed to air or CS after day 1 and day 3. Twenty five μg protein isolated from air-exposed or CS-exposed guinea pigs were converted, without any further treatment, to the DNP-derivative followed by immunoblotting as mentioned in Materials and Methods. 1 and 3 mean exposed to air (sham control) or CS for 1 day and 3 days, respectively. B, C, Histopathology profiles of guinea pig lung tissue sections after exposure to cigarette smoke for 3 days. B shows infiltration of inflammatory cells in the septal regions. C shows accumulation of leukocytes within the alveolar cells that are in all probability macrophages (indicated by → ; magnification × 20)