Table 1

Nitrite (μM); mean ± SEM


Control
 Concentration of peptide alone
 1 μg/ml LPS with stated concentration of peptides below


10 μM
 100 μM
 0 μM
 1 μM
 10 μM
 100 μM
MALY O1
 0.70
 2.00
 0.77
 44.6
 46.1
 47.2
 51.6

± 0.2
 ± 1.6
 ± 0.3
 ± 2.7
 ± 4.2
 ± 5.1
 ± 2.0
MALY 1O
 0.70
 0.87
 0.85
 44.6
 47.3
 40.3
 46.4

± 0.2
 ± 0.4
 ± 0.3
 ± 3.3
 ± 0.8
 ± 1.9
 ± 0.9
MALY II
 0.70
 0.64
 0.66
 44.6
 46.2
 43.4
 45.9

± 0.2
 ± 0.3
 ± 0.3
 ± 2.8
 ± 0.9
 ± 2.9
 ± 0.5

RAW cells were stimulated with medium alone (control), LPS (1 μg/ml) or various concentrations of the MALY TAT-linked PKC translocation inhibitor peptides (MALYO1 = cPKC inhibitor, MALY1O = PKC specific, MALYII = scrambled PKC inhibitor peptide), with or without LPS for 24 hr. NO production was assessed by Greiss assay. Results represent mean ± SEM (n= 3) and are representative of two separate experiments.

Jones et al. Journal of Inflammation 2007 4:23   doi:10.1186/1476-9255-4-23