Figure 6.

MMP Activity from synovial fluid of subjects with osteoarthritis and the inhibitory effects of progrado. Synovial fluid from subjects with active osteoarthritis was used as a source of matrix metalloproteinase gelatinolytic activity using gelatin zymography. Recombinant MMP-2 and MMP-9 was used for identification of gelatinolytic activity. Progrado was associated with a dose-dependent inhibition of both MMP-2 and MMP-9 activity. Complete inhibition of MMP-9 was evident at 4 μg/ml, with MMP-2 activity reduced by approximately 90%.