|
Results of lipoxygenase inhibitory effects of two representative alkaloids isolated from Mahonia aq. crude extract. |
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| Final concentration (mol/l) |
Activity of lipoxygenase |
% Inhibition |
IC50 (mol/L) |
|
|
|
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| Aqueous solution of jatrorrhizine (0.01M) |
||||
| 15.10-6 |
118.71 ± 4.37 |
22.05 ± 1.37 |
17.50.10-6 ± 1.27.10-6 |
|
| 20.10-6 |
23.89 ± 1.83 |
84.32 ± 3.74* |
||
| 25.10-6 |
12.73 ± 1.24 |
91.58 ± 2.91* |
||
| Control |
- |
152.29 ± 5.37 |
- |
|
| Aqueous solution of berberine (0.01M) |
||||
| 15.10-6 |
233.19 ± 7.41 |
14.88 ± 0.97 |
30.50.10-6 ± 2.87.10-6 |
|
| 20.10-6 |
213.40 ± 6.93 |
22.11± 1.24 |
||
| 25.10-6 |
157.02 ± 4.21 |
42.69 ± 2.38 |
||
| Control |
- |
273.96 ± 8.21 |
- |
|
|
Lipoxygenase activity was determined as absorbance increase at λmax = 234 nm at 3 minutes of incubation with or without inhibitor tested. Values of hydroperoxide content and lipoxygenase activity were calculated from equation c = A.V/ε.l.v, where A is the value of absorbance increase, V is the volume of incubation mixture, ε is the extinction coefficient for linolic acid (25.10-3 mol.l.cm-1), l is the length of the cuvette (1 cm) and v is the volume of enzyme (0.015 ml). Results are presented as percent of control ± SD, n = 3, * p < 0.05 vs. controls | ||||
Rackova et al. Journal of Inflammation 2007 4:15 doi:10.1186/1476-9255-4-15 |
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