Table 1

Results of lipoxygenase inhibitory effects of crude Mahonia extract and two representative alkaloid fractions tested.

Final concentration (g/L)
 Activity of lipoxygenase (kat)
 % Inhibition
 IC50 (g/L)
Crude Mahonia extract
 0.50.10-3
 7.40 ± 0.89
 43.03 ± 0.54
 0.76.10-3 ± 0.12.10-3

0.75.10-3
 6.34 ± 7.63
 51.19 ± 0.96*

1.25.10-3
 5.2 8 ± 0.78
 59.32 ± 1.39*

1.88.10-3
 3.53 ± 0.34
 72.80 ± 1.54*

2.50.10-3
 2.92 ± 0.28
 78.80 ± 1.72*
Control
 -
 12.99 ± 1.23
 -
Fraction I




1.25.10-3
 1.16 ± 0.11
 16.54 ± 7.67
 4.63.10-3 ± 1.03.10-3

2.50.10-3
 0.94 ± 0.09
 32.23 ± 2.63

3.75.10-3
 0.71 ± 0.09
 47.33 ± 2.24

5.10-3
 0.63 ± 0.07
 54.67 ± 1.64*

6.25.10-3
 0.55 ± 0.06
 60.43 ± 2.36*

7.50.10-3
 0.34 ± 0.04
 75.39 ± 2.10*
Control
 -
 1.39 ± 0.14
 -
Fraction II




1.25.10-3
 1.24 ± 0.12
 10.93 ± 4.76
 6.10.10-3 ± 0.98.10-3

2.50.10-3
 1.11 ± 0.10
 18.27 ± 6.23

3.75.10-3
 0.95 ± 0.09
 31.08 ± 2.25

5.10-3
 0.87 ± 0.09
 39.28 ± 2.47

6.25.10-3
 0.64 ± 0.07
 53.67 ± 2.33*

7.50.10-3
 0.56 ± 0.06
 60.86 ± 3.04*
Control
 -
 1.39 ± 0.24
 -

Lipoxygenase activity was determined as absorbance increase at λmax = 234 nm at 3 minutes of incubation with or without inhibitor tested. Values of hydroperoxide content and lipoxygenase activity were calculated from equation c = A.V/ε.l.v, where A is the value of absorbance increase, V is the volume of incubation mixture, ε is the extinction coefficient for linoleic acid (25.10-3 mol.l.cm-1), l is the length of the cuvette (1 cm) and v is the volume of enzyme (0.015 ml). Results are presented as percent of control ± SD, n = 3, * p < 0.05 vs. controls

Rackova et al. Journal of Inflammation 2007 4:15   doi:10.1186/1476-9255-4-15