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Open Access Research

Eosinophils in human oral squamous carcinoma; role of prostaglandin D2

Francis Davoine12*, Adrian Sim3, Charlie Tang1, Sibina Fisher4, Caroline Ethier1, Lakshmi Puttagunta3, Yingqi Wu1, W Tim McGaw5, Donald Yu5, Lisa Cameron1, Darryl J Adamko6 and Redwan Moqbel7

Author Affiliations

1 Pulmonary Research Group, University of Alberta, 559 Heritage Medical Research Centre, Edmonton, Alberta T6G 2S2, Canada

2 Campus-Saint-Jean, University of Alberta, 8406 Marie-Anne-Gaboury Street, Edmonton, Alberta T6C 4G9, Canada

3 Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada

4 Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada

5 Department of Dentistry, University of Alberta, Edmonton, Alberta, Canada

6 Department of Paediatrics, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

7 Department of Immunology, University of Manitoba, Winnipeg, Alberta, Canada

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Journal of Inflammation 2013, 10:4  doi:10.1186/1476-9255-10-4

Published: 31 January 2013

Abstract

Eosinophils are often predominant inflammatory leukocytes infiltrating oral squamous carcinoma (OSC) sites. Prostaglandins are secreted by oral carcinomas and may be involved in eosinophil infiltration. The objective of this study was to determine the factors contributing to eosinophil migration and potential anti-neoplastic effects on OSC. Eosinophil degranulation was evaluated by measuring release of eosinophil peroxidase (EPO). Eosinophil chemotaxis towards OSC cells was assessed using artificial basement membrane. Eosinophil infiltration was prominent within the tissue surrounding the OSC tumor mass. We observed growth inhibition of the OSC cell line, SCC-9, during co-culture with human eosinophils, in vitro, which correlated with EPO activity that possesses growth inhibitory activity. The PGD2 synthase inhibitor, HQL-79, abrogated migration towards SCC-9. Our data suggest that OSC-derived PGD2 may play an important role via CRTH2 (the PGD2 receptor on eosinophils) in eosinophil recruitment and subsequent anti-tumor activity through the action of eosinophil cationic proteins.

Keywords:
Eosinophils; Oral squamous carcinoma; Prostaglandin D2; HQL-79; Transmigration