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Open Access Research

XH-14, a novel danshen methoxybenzo[b]furan derivative, exhibits anti-inflammatory properties in lipopolysaccharide-treated RAW 264.7 cells

Geun-Mook Park1, Jong-Gab Jun2 and Jin-Kyung Kim1*

Author Affiliations

1 Department of Biomedical Science, Catholic University of Daegu, 330 Geumrak-Ri, Gyeoungsan-Si, 700-712, South Korea

2 Department of Chemistry and Institute of Natural Medicine, Hallym University, Chuncheon, 200-702, South Korea

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Journal of Inflammation 2013, 10:1  doi:10.1186/1476-9255-10-1

Published: 10 January 2013

Abstract

Background

XH-14 isolated from Salvia miltiorrhiza is a bioactive component and adenosine antagonist. In the present study, we evaluated anti-inflammatory properties of XH-14 in murine macrophages.

Methods

RAW 264.7 murine macrophage cell line was cultured with various concentrations of XH-14 in the absence or presence of lipopolysaccharide (LPS). LPS-induced release and mRNA expression of inflammatory mediators were examined by ELISA and real-time PCR. The modification of signal pathways involved in inflammatory reactions was determined by Western blotting analysis.

Results

XH-14 suppressed the generation of nitric oxide (NO) and prostaglandin E2, and the expression of inducible NO synthase and cyclooxygenase-2 induced by LPS. Similarly, XH-14 inhibited the release of pro-inflammatory cytokines induced by LPS in RAW 264.7 cells. The underlying mechanism of XH-14 on anti-inflammatory action was correlated with down-regulation of mitogen-activated protein kinase and activator protein-1 activation.

Conclusions

XH-14 inhibits the production of several inflammatory mediators and so might be useful for the treatment of various inflammatory diseases.

Keywords:
Inflammation; XH-14; Macrophage; Cytokines; AP-1